Tangential Flow Filtration Solutions

Pall's line of scalable TFF products range from single-use modules to cleanable cassettes, and from standard lab and benchtop systems to fully customizable process systems.

 

Develop tangential flow filtration applications using only a few milliliters of sample and scale up using the same membranes to pilot and full scale processes in excess of 10,000 liters. Our supporting services ease the implementation of TFF technologies into your processes through training, trials, validation, and technical support. Pall partners with our customers to design the best products to address each purification challenge.    

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Automated Systems

Scalable, pre-designed systems and custom-designed systems to suit your specific process needs.
Scalable, pre-designed systems and custom-designed systems to suit your specific process needs.
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Featured Product: Cadence™ Single-Pass Tangential Flow Filtration Modules and Systems

Eliminate the Recirculation Loop and Streamline Operations to Optimize Downstream Processing. Cadence Single-Pass TFF ( SPTFF ) is a patented breakthrough technology that allows direct flow-through concentration with no recirculation of product. SPTFF enables high concentration of shear sensitive proteins and antibodies >160 grams/liter. 

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Cassette Holders

Designed for optimum performance and product recovery Pall TFF cassette holders are available in a variety of sizes to meet every application requirement.
Designed for optimum performance and product recovery Pall TFF cassette holders are available in a variety of sizes to meet every application requirement.
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Featured Product: Tangential Flow Filtration Membrane Cassette Holders

TFF cassette holders for the medium and large-scale production environment have a small footprint, yet still are capable of holding large areas of membrane.   All stainless steel holders are engineered to precise specifications with a high standard internal and external surface finish and are available with either manual torque (MT) or auto torque (AT) compression systems for ease of use.     

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Cassettes

The proven T-Series Cassette design optimizes your process economics.
The proven T-Series Cassette design optimizes your process economics.
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Featured Product: T Series Membrane Cassettes

High performance Omega PES membrane combined with significant material and design improvements deliver highly reliable fluid dynamics and better process economics.

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Hollow Fiber Modules

Pall's Microza♦ polymeric hollow fiber membranes are made from durable fibers available in a wide variety of ratings, polymers and sizes.
Pall's Microza♦ polymeric hollow fiber membranes are made from durable fibers available in a wide variety of ratings, polymers and sizes.
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Featured Product: Microza♦ Membranes - Polysulfone

Microza♦ ultrafiltration membranes are unique in their construction. Every membrane has an asymmetric structure,with a membrane layer both on the inside, and on the outside of the fiber, overlaying a core comprising a very open support for excellent flow characteristics, and a central strengthening layer for outstanding fiber durability in long-term service.

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Single-Use Tangential Flow Filtration

A simpler, easier and safer single-use TFF solution for R&D, clinical or commercial manufacturing.
A simpler, easier and safer single-use TFF solution for R&D, clinical or commercial manufacturing.
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Featured Product: Allegro™ CM150 Single-Use Tangential Flow Filtration System

Optimized small scale SUTFF processing.  The Allegro CM150 single-use tangential flow filtration (SUTFF) system is easy to use and offers all the critical sensors for running ultrafiltration (UF) and diafiltration (DF) processes at 2-20 L batch volumes.    

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Allegro Single-Use Tangential Flow (SUTFF) Systems

Allegro Single-Use Tangential Flow (SUTFF) Systems

The Allegro SUTFF systems range offers scalable technology for 2 L to 2000 L batches, using 0.1 to 10 m2. cassette area. These systems offer fully single–use processing for ultrafiltration and diafiltration, with a choice of single–use sensors, intuitive control system, and excellent system design to ensure an excellent user experience. 

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Rapid and Efficient

Tangential Flow Filtration Introduction

Tangential Flow Filtration (TFF), also called Cross Flow Filtration (CFF), is a rapid and efficient method for filtration and separation of solutions containing biomolecules, or particles such as viruses, bacteria or cellular material. It is a process whereby product flow (feed) is directed tangentially along the surface of a membrane with most of the solution circulated back to the feed tank.

 

The rapid flow of feed solution across the membrane acts to 'sweep' the surface, reducing concentration polarization (product concentration at the membrane surface). It also prevents build-up of foulants that can plug the pores at the membrane surface. The rapid cross flow creates a pressure drop, which forces some of the feed solution and dissolved molecules that are smaller than the pores in the membrane, through the membrane filter. The solution that passes through the membrane is referred to as filtrate or permeate. Molecules or particles larger than the membrane pores are retained in the feed solution and effectively concentrated.

 

Membrane filtration can be classified as either a microfiltration (MF) or ultrafiltration (UF) process. Microfiltration membranes, with pore sizes typically between 0.1 micron and 1 micron, are generally used for clarification, sterilization and removal of micro-particulates or for cell harvesting. Ultrafiltration membranes, with much smaller pore sizes between 0.001 and 0.1 micron, are used for concentrating and desalting dissolved molecules (protein, peptides, nucleic acids, carbohydrates and other biomolecules), exchanging buffers, fractionation and water purification. Ultrafiltration membranes are typically classified by Molecular Weight Cut Off (MWCO), rather than pore size.

 

The fluid dynamics of cross flow filtration reduces membrane fouling and maintains filtration rates (flux) for a longer period of use, thus increasing membrane throughput (greater capacity) compared to traditional cartridge (direct flow) filtration. Additional advantages of TFF include the ability to reuse the filter modules and relatively low capital costs.

 

TFF can be applied to a wide range of biological fields. It is applicable to R&D discovery applications right through pilot and full-scale production. TFF can be used to concentrate and desalt sample solutions ranging in volume from a few milliliters to thousands of liters. It can be used to fractionate large from small biomolecules, harvest cell suspensions and clarify fermentation broths and cell lysate.

 

TFF Applications

 

Microfiltration and ultrafiltration processes incorporating tangential flow or cross flow filtration is utilized in a wide range of biopharmaceutical applications. Examples of a few typical applications are listed below:

Concentration and desalting of protein, peptide, and oligonucleotide solutions

Purification and recovery of antibodies or recombinant proteins

Vaccine and conjugate concentration and diafiltration.

Fractionation of protein mixtures

Blood plasma fractionation and purification

Cell broth clarification, concentration

Cell culture perfusion such as in monoclonal antibody (mAb) production

Clarification of Fermentation broths

Concentration and washing of bacterial cells

Water and buffer purification (endotoxin removal)    

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